Title

Homogeneous and Heterogeneous Tertiary Structure Ensembles of Amyloid-β Peptides

Document Type

Article

Publication Date

2011

Abstract

The interplay of modern molecular simulation and high-quality nuclear magnetic resonance (NMR) experiments has reached a fruitful stage for quantitative characterization of structural ensembles of disordered peptides. Amyloid-β 1–42 (Aβ42), the primary peptide associated with Alzheimer’s disease, and fragments such as Aβ21–30 are both classified as intrinsically disordered peptides (IDPs). We use a variety of NMR observables to validate de novo molecular dynamics simulations in explicit water to characterize the tertiary structure ensemble of Aβ42 and Aβ21–30 from the perspective of their classification as IDPs. Unlike the Aβ21–30 fragment that conforms to expectations of an IDP that is primarily extended, we find that Aβ42 samples conformations reflecting all possible secondary structure categories and spans the range of IDP classifications from collapsed structured states to highly extended conformations, making it an IDP with a far more heterogeneous tertiary ensemble.

Published In

Biochemistry

Volume

50

Issue

35

Pages

7612-7628

DOI

10.1021/bi200732x

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