Document Type
Article
Publication Date
2013
Published In
Nature Communications
Volume
4
Pages
1697
DOI
10.1038/ncomms2682
Recommended Citation
Sauguet, L.; Howard R., J.; Malherbe, L.; Lee Ui, S.; Corringer, P.-J.; Harris, R. A.; Delarue, M., Structural basis for potentiation by alcohols and anaesthetics in a ligand-gated ion channel. Nat. Commun. 2013, 4, 1697.
Abstract
Ethanol alters nerve signalling by interacting with proteins in the central nervous system, particularly pentameric ligand-gated ion channels. A recent series of mutagenesis experiments on Gloeobacter violaceus ligand-gated ion channel, a prokaryotic member of this family, identified a single-site variant that is potentiated by pharmacologically relevant concentrations of ethanol. Here we determine crystal structures of the ethanol-sensitized variant in the absence and presence of ethanol and related modulators, which bind in a transmembrane cavity between channel subunits and may stabilize the open form of the channel. Structural and mutagenesis studies defined overlapping mechanisms of potentiation by alcohols and anaesthetics via the inter-subunit cavity. Furthermore, homology modelling show this cavity to be conserved in human ethanol-sensitive glycine and GABA(A) receptors, and to involve residues previously shown to influence alcohol and anaesthetic action on these proteins. These results suggest a common structural basis for ethanol potentiation of an important class of targets for neurological actions of ethanol.
